Plasmid

Part:BBa_K1369011:Design

Designed by: Monique Alkiewicz   Group: iGEM14_Caltech   (2014-10-09)


pCon LamC, constituatively expressed non-inhibited HK with 4 SH3 domains


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 1346
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 86
    Illegal XbaI site found at 2630
    Illegal XbaI site found at 4656
    Illegal SpeI site found at 7
    Illegal PstI site found at 2636
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 86
    Illegal NheI site found at 42
    Illegal SpeI site found at 7
    Illegal SpeI site found at 1347
    Illegal PstI site found at 1361
    Illegal PstI site found at 2636
    Illegal NotI site found at 92
    Illegal NotI site found at 1354
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 86
    Illegal BglII site found at 80
    Illegal BamHI site found at 2237
    Illegal XhoI site found at 2246
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 1347
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 86
    Illegal XbaI site found at 2630
    Illegal XbaI site found at 4656
    Illegal SpeI site found at 7
    Illegal PstI site found at 2636
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 86
    Illegal EcoRI site found at 1
    Illegal XbaI site found at 2630
    Illegal XbaI site found at 4656
    Illegal SpeI site found at 7
    Illegal SpeI site found at 1347
    Illegal PstI site found at 1361
    Illegal PstI site found at 2636
    Illegal AgeI site found at 579
    Illegal AgeI site found at 3900
    Illegal AgeI site found at 4224
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2345
    Illegal BsaI site found at 2523
    Illegal SapI.rc site found at 1532
    Illegal SapI.rc site found at 1742
    Illegal SapI.rc site found at 1952
    Illegal SapI.rc site found at 2162


Design Notes

It contains 4 SH3 domains to be used in the scaffold system


Source

Lactobacillus plantarum WCFS1

References